文章基本信息

标题：The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus
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作者：Lilis Nurjanah ; Sri Nuryati ; Alimuddin Alimuddin 等
期刊名称：Jurnal Akuakultur Indonesia
印刷版ISSN：1412-5269
出版年度：2018
卷号：17
期号：2
页码：147-157
DOI：10.19027/jai.17.2.147-157
语种：English
出版社：Jurnal Akuakultur Indonesia
其他摘要：ABSTRACT Streptococcosis caused by Streptococcus agalactiae bacteria, is a type of disease that often found in Nile tilapia farming. This study was performed to determine the effectiveness of the concentration and frequency of giving chopped banana stem as the immunostimulant to stimulate the non‒specific immune system of tilapia against S. agalactiae infection. This study used factorial completely randomized design, consisted of two factors: concentration and changing frequency (replacement time interval) of banana stem on Nile tilapia rearing media with 11 treatment and each treatment was given three replications. The concentration of 5 g/L, 10 g/L and 15 g/L and the three days, seven days and no replacement time interval were used in this study. The immersion treatment with the chopped banana stem was done for 14 days, then the challenge test with S. agalactiae was on the 15th day for 14 days observation. The result showed that chopped banana stem contained active compounds of alkaloids, namely: 17.63% flavonoids, 0.02% tannin and 0.24% saponins. This active compounds could increase the non-specific immune system including respiratory burst , lysozyme activity, phagocyte activity, erythrocyte, leucocyte and haemoglobin. Treatment with a concentration of 5 g/L and no replacement of the chopped banana stem was the best treatment with 75% relative percent survival, highest than other treatments. In addition, it also has highest phagocytes (36.3%), respiratory burst (0.58 at O.D. 630), and lysozyme activity (72.7 unit/mL) after four days challenged with S. agalactiae.
其他关键词：Banana stem; immunostimulant; Nile tilapia; non-specific immune system; Streptococcus agalatiae