文章基本信息

标题：Residential Proximity to Major Roadways at Birth, DNA Methylation at Birth and Midchildhood, and Childhood Cognitive Test Scores: Project Viva(Massachusetts, USA)
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作者：Cheng Peng ; Martijn den Dekker ; Andres Cardenas 等
期刊名称：Environmental Health Perspectives
印刷版ISSN：0091-6765
电子版ISSN：1552-9924
出版年度：2018
卷号：126
期号：9
页码：97006
DOI：10.1289/EHP2034
语种：English
出版社：OCR Subscription Services Inc
摘要：Background: Epigenetic variability is hypothesized as a regulatory pathway through which prenatal exposures may influence child development and health. Objective: We sought to examine the associations of residential proximity to roadways at birth and epigenome-wide DNA methylation. We also assessed associations of differential methylation with child cognitive outcomes. Methods: We estimated residential proximity to roadways at birth using a geographic information system (GIS) and cord blood methylation using Illumina’s HumanMethylation450-array in 482 mother–child pairs in Project Viva. We identified individual CpGs associated with residential-proximity-to-roadways at birth using robust linear regression [ false discovery rate ( FDR ) < 0.05 ]. We also estimated association between proximity-to-roadways at birth and methylation of the same sites in blood samples collected at age 7–11 y ( N = 415 ). We ran the same analyses in the Generation R Study for replication ( N = 641 ). In Project Viva, we investigated associations of differential methylation at birth with midchildhood cognition using linear regression. Results: Living closer to major roadways at birth was associated with higher cord blood (and—more weakly—midchildhood blood) methylation of four sites in LAMB2 . For each halving of residential-proximity-to-major-roadways, we observed a 0.82% increase in DNA methylation at cg05654765 [95% confidence interval (CI): (0.54%, 1.10%)], 0.88% at cg14099457 [95% CI: (0.56%, 1.19%)], 0.19% at cg03732535 [95% CI: (0.11%, 0.28)], and 1.08% at cg02954987 [95% CI: (0.65%, 1.51%)]. Higher cord blood methylation of these sites was associated with lower midchildhood nonverbal cognitive scores. Our results did not replicate in the Generation R Study. Conclusions: Our discovery results must be interpreted with caution, given that they were not replicated in a separate cohort. However, living close to major roadways at birth was associated with cord blood methylation of sites in LAMB2 —a gene known to be linked to axonal development—in our U.S. cohort. Higher methylation of these sites associated with lower nonverbal cognitive scores at age 7–11 y in the same children.