文章基本信息

标题：LIMITING FACTORS IN SINGLE PARTICLE CRYO ELECTRON TOMOGRAPHY
本地全文：下载
作者：Mikhail Kudryashev ; Daniel Castaño-Díez ; Henning Stahlberg 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2012
卷号：1
期号：2
DOI：10.5936/csbj.201207002
语种：
出版社：Computational and Structural Biotechnology Journal
摘要：Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements within tomograms allow exploring their structures within the native context of the cell, avoiding the need for protein isolation and purification. Today, many different data acquisition protocols and software solutions are available to researchers to determine average structures of macromolecular complexes and potentially to classify structural intermediates. Here, we list the density maps reported in the literature, and analyze each structure for the chosen instrumental settings, sample conditions, main processing steps, and obtained resolution. We present conclusions that identify factors currently limiting the resolution gained by this approach.