文章基本信息

标题：LC-MS METABOLOMICS FROM STUDY DESIGN TO DATA-ANALYSIS – USING A VERSATILE PATHOGEN AS A TEST CASE
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作者：Maya Berg ; Manu Vanaerschot ; Andris Jankevics 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2013
卷号：4
期号：5
DOI：10.5936/csbj.201301002
语种：
出版社：Computational and Structural Biotechnology Journal
摘要：Thanks to significant improvements in LC-MS technology, metabolomics is increasingly used as a tool to discriminate the responses of organisms to various stimuli or drugs. In this minireview we discuss all aspects of the LC-MS metabolomics pipeline, using a complex and versatile model organism, Leishmania donovani, as an illustrative example. The benefits of a hyphenated mass spectrometry platform and a detailed overview of the entire experimental pipeline from sampling, sample storage and sample list set-up to LC-MS measurements and the generation of meaningful results with state-of-the-art data-analysis software will be thoroughly discussed. Finally, we also highlight important pitfalls in the processing of LC-MS data and comment on the benefits of implementing metabolomics in a systems biology approach.
关键词：mass spectrometry ; HILIC ; Leishmania ; unicellular trypanosomatid parasites ; global molecular profiles ; systems biology