文章基本信息

标题：Specific Light-Up System for Protein and Metabolite Targets Triggered by Initiation Complex Formation
本地全文：下载
作者：Hiroto Fujita ; Yuka Kataoka ; Remi Nagano 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
页码：15191
DOI：10.1038/s41598-017-15697-8
语种：English
出版社：Springer Nature
摘要：Gene regulation systems are mimicked by simple quantitative detection of non-nucleic acid molecular targets such as protein and metabolite. Here, we describe a one-tube, one-step real-time quantitative detection methodology for isothermal signal amplification of those targets. Using this system, real-time quantitative detection of thrombin and streptomycin, which were used as examples for protein and metabolite targets, was successfully demonstrated with detection limits of at most 50 pM and 75 nM, respectively. Notably, the dynamic range of target concentrations could be obtained for over four orders of magnitude. Thus, our method is expected to serve as a point-of-care or on-site test for medical diagnosis and food and environmental hygiene.