文章基本信息

标题：Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks
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作者：Pietro Roversi ; Steven Johnson ; Stephen G. Preston 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
页码：16057
DOI：10.1038/s41598-017-16413-2
语种：English
出版社：Springer Nature
摘要：Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology to Japanin are also likely to bind cholesterol or cholesterol derivatives.