文章基本信息

标题：Multiple mutations and overexpression of the MdaE7 carboxylesterase gene associated with male-linked malathion resistance in housefly, Musca domestica (Diptera: Muscidae)
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作者：Yi Zhang ; Jing Li ; Zhuo Ma 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：224
DOI：10.1038/s41598-017-17325-x
语种：English
出版社：Springer Nature
摘要：-Thr), not found in susceptible houseflies, revealing a strong correlation between these mutations and the development of malathion resistance. Further genetic analysis conducted with bioassays by topical application and nucleotide polymorphism detection provided a first line of molecular evidence for a linkage between a male-determining factor and MdαE7 gene in the MSS and N-MRS males. This linkage results in a much higher level of malathion resistance for males than females in the MSS strain. Lastly, quantitative real-time PCR showed that MdαE7 was over expressed in the resistant strain due to the increased transcription level of mRNA rather than gene duplication.