文章基本信息

标题：Real-time TIRF observation of vinculin recruitment to stretched α-catenin by AFM
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作者：Koichiro Maki ; Sung-Woong Han ; Yoshinori Hirano 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：1575
DOI：10.1038/s41598-018-20115-8
语种：English
出版社：Springer Nature
摘要：domain), modified on coverslips, were stretched by AFM and their recruitment of Alexa-labeled full-length vinculin molecules, dissolved in solution, were observed simultaneously, in real time, using TIRF. We applied a physiologically possible range of tensions and extensions to α-catenin and directly observed its vinculin recruitment. Our new system could be used in the fields of mechanobiology and biophysics to explore functions of proteins under tension by coupling biomechanical and biochemical information.