文章基本信息

标题：Red fluorescent cAMP indicator with increased affinity and expanded dynamic range
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作者：Yusaku Ohta ; Toshiaki Furuta ; Takeharu Nagai 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：1866
DOI：10.1038/s41598-018-20251-1
语种：English
出版社：Springer Nature
摘要：= 0.3 μM) and expanded dynamic range (860% at pH 7.2) allowed the detection of subtle changes in the cellular cAMP dynamics at sub-μM concentrations, which could not be easily observed with existing indicators. Increased detection sensitivity also strengthened the advantages of using R-FlincA as a red fluorescent indicator, as it permits a series of applications, including multi-channel/function imaging of multiple second messengers and combinatorial imaging with photo-manipulation. These results strongly suggest that R-FlincA is a promising tool that accelerates cAMP research by revealing unobserved cAMP dynamics at a low concentration range.