文章基本信息

标题：Thermal stabilization of the deglycating enzyme Amadoriase I by rational design
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作者：Federica Rigoldi ; Stefano Donini ; Francesca Giacomina 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：3042
DOI：10.1038/s41598-018-19991-x
语种：English
出版社：Springer Nature
摘要：(55.3 °C and 60.6 °C, respectively) compared to the wild-type enzyme (52.4 °C). Moreover, SS17 shows clear hyperstabilization, with residual activity up to 95 °C, whereas the wild-type enzyme is fully inactive at 55 °C. Our computational screening method can therefore be considered as a promising approach to expedite the design of thermostable enzymes.