文章基本信息

标题：Directional preparation of anticoagulant-active sulfated polysaccharides from Enteromorpha prolifera using artificial neural networks
本地全文：下载
作者：Jiefen Cui ; Yinping Li ; Shixin Wang 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：3062
DOI：10.1038/s41598-018-21556-x
语种：English
出版社：Springer Nature
摘要：The sulfated polysaccharides from Enteromorpha prolifera (PE) are a potential source of anticoagulant agents. In this study, the PE was degraded by specific degradase and five hydrolysis products with different molecular weights were prepared. The product of 206 kDa is a kind of high rhamnose-containing polysaccharide with sulfate ester (34.29%). It could effectively prolong the activated partial thromboplastin time (APTT), which indicated inhibition of the intrinsic coagulation pathway. The artificial neural network (ANN) was built to realize the directional preparation of anticoagulant-active polysaccharides. Based on monitoring glucose concentration on-line, a visualization system of enzymatic hydrolysis was developed to simplify the operation of ANN. The model could be further applied to predict molecular weights of polysaccharides that possess diverse biological activities.