文章基本信息

标题：Adult mouse eIF2Bε Arg191His astrocytes display a normal integrated stress response in vitro
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作者：Lisanne E. Wisse ; Timo J. ter Braak ; Malu-Clair van de Beek 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：3773
DOI：10.1038/s41598-018-21885-x
语种：English
出版社：Springer Nature
摘要：), representative of the human disease. Neuropathological examination of VWM patient and mouse brain tissue suggests that astrocytes are primarily affected. We hypothesized that VWM astrocytes are selectively hypersensitive to ISR induction, resulting in a heightened response. We cultured astrocytes from wildtype and VWM mice and investigated the ISR in assays that measure transcriptional induction of stress genes, protein synthesis rates and cell viability. We investigated the effects of short- and long-term stress as well as stress recovery. We detected congruent results amongst the various assays and did not detect a hyperactive ISR in VWM mouse astrocytes.