文章基本信息

标题：Tunable Ampere phase plate for low dose imaging of biomolecular complexes
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作者：Amir H. Tavabi ; Marco Beleggia ; Vadim Migunov 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：5592
DOI：10.1038/s41598-018-23100-3
语种：English
出版社：Springer Nature
摘要：A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.