文章基本信息

标题：Substrate specificity of human MCPIP1 endoribonuclease
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作者：Mateusz Wilamowski ; Andrzej Gorecki ; Marta Dziedzicka-Wasylewska 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：7381
DOI：10.1038/s41598-018-25765-2
语种：English
出版社：Springer Nature
摘要：and the RNA targets were approximately 10 nM. Interestingly, we observed that the presence of a zinc finger in the PIN domain increases the affinity of this protein fragment to 25-nucleotide-long stem-loop RNA but not to shorter ones. Furthermore, size exclusion chromatography of the MCPIP1 and PIN proteins suggested that MCPIP1 undergoes homooligomerization during interaction with RNA substrates. Our results provide insight into the mechanism of MCPIP1 substrate recognition and its affinity towards various oligonucleotides.