文章基本信息

标题：Synthesis and Characterization of a Nano-Polyplex system of GNRs-PDMAEA-pDNA: An Inert Self-Catalyzed Degradable Carrier for Facile Gene Delivery
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作者：Ali Dinari ; Tahereh Tohidi Moghadam ; Mahdi Abdollahi 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：8112
DOI：10.1038/s41598-018-26260-4
语种：English
出版社：Springer Nature
摘要：H NMR, gel permeation chromatography (GPC), Fourier transform-infrared (FT-IR) spectroscopy, Zeta potential analyzer and transmission electron microscopy (TEM). Features of higher transfection and lower toxicity compared to the previously reported polyplex of PDMAEA, as well as the gold standard PEI, have been shown in all molecular weights and defined N/P ratios (10-200). The ideal physicochemical properties for escaping from the cell barriers, covering the large volume of genetic material (pDNA) and high efficiency of loading polyplexes on GNRs' surface make it an ideal carrier. The results of this effort pave way in designing a new generation of nanoparticle-based delivery systems for nucleic acid therapy and gene editing.