文章基本信息

标题：A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
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作者：Elizabeth Evans ; Romain Paillot ; María Rocío López-Álvarez 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：8237
DOI：10.1038/s41598-018-26512-3
语种：English
出版社：Springer Nature
摘要：The 3Rs principles (Replacement, Reduction and Refinement) are focused on finding alternatives to the use of animals in research. In this regard, cell lines are popular and useful tools for the replacement of primary cells in in vitro studies. However, around 15-30% of cell lines used in research have been misidentified or cross-contaminated generating concerns about the results obtained from experiments that use them. Here we described how old aliquots of an equine macrophage cell line (e-CAS) stored at the Animal Health Trust did not contain equine cells but macrophages of murine origin (m-CAS).