文章基本信息

标题：In planta proximity dependent biotin identification (BioID)
本地全文：下载
作者：Madiha Khan ; Ji-Young Youn ; Anne-Claude Gingras 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：9212
DOI：10.1038/s41598-018-27500-3
语种：English
出版社：Springer Nature
摘要：Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.