文章基本信息

标题：Quantitative assessment of individual populations within polymicrobial biofilms
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作者：Susana Patrícia Lopes ; Nuno Filipe Azevedo ; Maria Olívia Pereira 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：9494
DOI：10.1038/s41598-018-27497-9
语种：English
出版社：Springer Nature
摘要：) compared to culture. But the discrepancies between PNA-FISH and q-PCR data (eg D. pigrum limited assessment by q-PCR) demonstrate the effect of biofilm heterogeneity in method's reliability. As the heterogeneity in biofilms is a reflection of a myriad of variables, tailoring an accurate picture of communities´ changes is crucial. This work demonstrates that at least two, but preferentially three, quantification techniques are required to obtain reliable measures and take comprehensive analysis of polymicrobial biofilm-associated infections.