文章基本信息

标题：Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors
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作者：Dorothee Eicke ; Anja Baigger ; Kai Schulze 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：10146
DOI：10.1038/s41598-018-28459-x
语种：English
出版社：Springer Nature
摘要：MKs per 50 ml medium. Laminin-coated microcarriers increased MK production per iPSC by up to 10-fold. MKs obtained in this system showed typical features of mature MKs and were able to produce PLTs in vitro and in vivo. To increase safety, MKs produced in the bioreactors were irradiated; a procedure that did not affect their capability to form proPLTs and PTLs after transfusion. In vitro generated MKs represent a promising alternative to donor PLTs and open the possibility for the development of innovative MK-based cell therapies.