文章基本信息

标题：Downstream Products are Potent Inhibitors of the Heparan Sulfate 2-O-Sulfotransferase
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作者：David F. Thieker ; Yongmei Xu ; Digantkumar Chapla 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：11832
DOI：10.1038/s41598-018-29602-4
语种：English
出版社：Springer Nature
摘要：values correlate simply with degree of oligosaccharide sulfation. Structural analysis predicts a mode of inhibition in which 6-O-sulfate groups located on glucosamine residues present in highly-sulfated oligosaccharides occupy the canonical binding site of the nucleotide cofactor. The unexpected finding that oligosaccharides associated with later stages in HS biosynthesis inhibit HS2ST indicates that the enzyme must be separated temporally and/or spatially from downstream products during biosynthesis in vivo, and highlights a challenge for the enzymatic synthesis of lengthy HS chains in vitro.