文章基本信息

标题：A simple and inexpensive quantitative technique for determining chemical sensitivity in Saccharomyces cerevisiae
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作者：Chao-Wei Hung ; Jorge Y. Martínez-Márquez ; Fatima T. Javed 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：11919
DOI：10.1038/s41598-018-30305-z
语种：English
出版社：Springer Nature
摘要：) using common laboratory equipment. We demonstrate the utility of this method using chemicals commonly used to monitor changes in membrane traffic. When compared to traditional agar-based plating methods, this method is more sensitive and can detect defects not apparent using other protocols. Additionally, this method reduces the experimental protocol from five days to 18 hours for the toxic amino acid canavanine. Furthermore, this method provides reliable results using lower amounts of chemicals. Finally, this method is easily adapted to additional chemicals as demonstrated with an engineered system that activates the spindle assembly checkpoint in response to rapamycin with differing efficiencies. This approach provides researchers with a cost-effective method to perform chemical genetic profiling without specialized equipment.