文章基本信息

标题：Accelerated pharmaceutical protein development with integrated cell free expression, purification, and bioconjugation
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作者：Dominique Richardson ; Jaakko Itkonen ; Julia Nievas 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：11967
DOI：10.1038/s41598-018-30435-4
语种：English
出版社：Springer Nature
摘要：fusion tag coupled with Ni-NTA). The latter was more efficient, but more time consuming. Light triggered release was clearly demonstrated. Moreover, we transferred biotin from the capture peptide to the target protein without further purification steps. Finally, the target protein was released in a buffer-volume and composition of our choice, omitting the need for protein concentration or changing the buffer. Split-intein mediated capture, protein trans splicing followed by light triggered release, and bioconjugation for proteins synthesized in cell free systems might be performed in an integrated workflow resulting in the fast production of the target protein.