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  • 标题:Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene
  • 本地全文:下载
  • 作者:Noa Aloush ; Tomer Schvartz ; Andres I. König
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2018
  • 卷号:8
  • 期号:1
  • 页码:14527
  • DOI:10.1038/s41598-018-32824-1
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:population. This enabled us to improve live cell imaging of bioorthogonally labelled intracellular proteins, and to simultaneously label two different proteins in a cell. Our results indicate that the number of introduced PylT genes can be minimized according to the transfected cell line, incorporated ncAA, and application.
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