文章基本信息

标题：Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda)
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作者：Kahina Mehennaoui ; Sylvain Legay ; Tommaso Serchi 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：15225
DOI：10.1038/s41598-018-33561-1
语种：English
出版社：Springer Nature
摘要：, silver nanoparticles (AgNPs) 40 nm and gold nanoparticles (AuNPs) 40 nm. Effects on HSP90 were evaluated and data normalized using Clathrin and SDH. A down-regulation of HSP90 was observed when G. fossarum were exposed to AuNPs 40 nm whereas no effects were observed when G. fossarum were exposed to AgNPs 40 nm. This study highlights the importance of the preliminary determination of suitable reference genes for RT-qPCR experiments. Additionally, this study allowed, for the first time, the determination of a set of valuable genes that can be used in other RT-qPCR studies using G. fossarum as model organism.