文章基本信息

标题：Artificially designed hybrids facilitate efficient generation of high-resolution linkage maps
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作者：Kazutoshi Yoshitake ; Yoji Igarashi ; Misaki Mizukoshi 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：16104
DOI：10.1038/s41598-018-34431-6
语种：English
出版社：Springer Nature
摘要：When sequencing eukaryotic genomes, linkage maps are indispensable for building scaffolds to assemble and/or to validate chromosomes. However, current approaches to constructing linkage maps are limited by marker density and cost-effectiveness, especially for wild organisms. We have now devised a new strategy based on artificially generated hybrid organisms to acquire ultrahigh-density genomic markers at reduced cost and build highly accurate linkage maps. We have also developed the novel analysis pipeline Scaffold Extender with Low Depth Linkage Analysis (SELDLA) for data processing to generate linkage maps and draft genomes. Using SELDLA, linkage maps and improved genomes for two species of pufferfish, Takifugu rubripes and Takifugu stictonotus, were obtained simultaneously. The strategy is applicable to a wide range of sexually reproducing organisms, and could, therefore, accelerate the whole genome analysis of various organisms including fish, mollusks, amphibians, insects, plants, and even mammals.