首页    期刊浏览 2024年12月06日 星期五
登录注册

文章基本信息

  • 标题:Rapid detection of microbial contamination in UHT milk: practical application in dairy industry
  • 本地全文:下载
  • 作者:Martina Kračmarová ; Hana Stiborová ; Šárka Horáčková
  • 期刊名称:Czech Journal of Food Sciences
  • 印刷版ISSN:1212-1800
  • 电子版ISSN:1805-9317
  • 出版年度:2018
  • 卷号:36
  • 期号:5
  • 页码:357-364
  • DOI:10.17221/24/2018-CJFS
  • 出版社:Czech Academy of Agricultural Sciences
  • 摘要:Microbial quality of ultra-high temperature (UHT) milk is usually ascertained by a total bacterial count (TBC) cultivation. But this is time consuming, so there is a tendency to search for faster and simpler methods. We compared three instruments, focusing on shortening the detection time and their suitability for practical use in dairy plants. Two of them, RapiScreen Dairy 1000 and Promilite III, detect microbial contamination by measuring adenosine triphosphate bioluminescence; the third, GreenLight, is based on oxygen consumption analysis. In the laboratory experiments, samples of UHT milk, were spiked with low concentration of microorganisms and then the level of microbial contamination was evaluated using the above-mentioned instruments together with cultivation method as a control. The instruments were also applied in a dairy plant to test 182 real samples. All investigated methods determined microbial quality faster than the TBC, but in some cases false positive and false negative results were obtained. Therefore, precise testing including optimizing pre-incubation time for bacteria enrichment is needed prior to industrial use.
  • 关键词:ATP bioluminescence; oxygen-consumption analysis; total bacterial count; milk contamination
国家哲学社会科学文献中心版权所有