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  • 标题:Nucleoprotein vaccine induces cross-protective cytotoxic T lymphocytes against both lineages of influenza B virus
  • 作者:Lee, So-Young ; Lee, So-Young ; Kang, Jung-Ok
  • 期刊名称:Clinical and Experimental Vaccine Research
  • 印刷版ISSN:2287-3651
  • 出版年度:2019
  • 卷号:8
  • 期号:1
  • 页码:54-63
  • DOI:10.7774/cevr.2019.8.1.54
  • 语种:English
  • 出版社:KoreaMed Synapse
  • 摘要:Purpose

    The influenza B virus diverges into two antigenically distinct lineages: B/Yamagata and B/Victoria. Influenza B is the dominant circulating virus during some influenza seasons, and recent data demonstrated that influenza A and B infection similarly cause severe clinical symptoms in hospitalized patients. Nucleoprotein (NP) is a good target for a universal influenza vaccine. This study investigated whether NP epitope variation within two lineages affects the dominant cytotoxic T lymphocyte (CTL) responses induced by vaccination and the resultant protective immunity.

    Materials and Methods

    The NP of B/Yamagata/16/1988, the representative strain of the Yamagata lineage, includes a dominant CTL epitope, FSPIRITFL, while B/Shangdong/7/1997 from the Victoria lineage has one amino acid difference in this sequence, FSPIRVTFL. Two recombinant replication-deficient adenovirus (rAd)-vectored vaccines expressing either NP were prepared (rAd/B-NP(I) and rAd/B-NP(V), respectively) and administered to BALB/c mice intranasally. To examine the efficacy of vaccination, antibody responses, CTL responses, and morbidity/mortality after challenge were measured.

    Results

    Both vaccines induce similar antibody and CD8 T-cell responses cross-reacting to both epitopes, and also confer cross-protection against both lineages regardless of amino acid difference.

    Conclusion

    The rAd-vectored vaccine expressing the NP could be developed as universal influenza B vaccine which provides broader protection.

  • 关键词:Influenza B Virus; Cross protective immunity; Nucleoproteins; cytotoxic T lymphocytes; Recombinant Adenovirus; Epitope
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