标题:Effectivity of prebiotic mannan oligosaccharides as the immunity enhancer and growth response on whiteleg shrimp Litopenaeus vannamei against white spot disease
其他摘要:ABSTRACT This study aimed to evaluate the immune response and growth performance of white shrimp administered with prebiotic mannan oligosaccharides (MOS) with dosages of (0%, 0.2%, 0.4%, and 0.8% in diet) and used in the feeding trial. Shrimps ( Litopenaeus vannamei ) (the initial average weight was 3.416±0.064 g) were fed at satiation, three times a day. A completely randomized design was used in the study. Shrimps were cultured at the stock density of 15 shrimps 40/L for each treatment in triplicates. After 30 days of the feeding trial, shrimp were challenged with white spot syndrome virus filtrate by intramuscular injection. The total gut bacteria, total haemocyte count (THC), phenoloxydase (PO), and respiratory burst (RB) activity were observed 4 times, before the experiment, day 30 th before challenge test, day 32 nd after challenge test, and day 36 th the end of the experiment. The shrimp survival was observed at day 36 th to evaluate the immune responses. The results showed that THC, PO activity, RB activity, growth performance, and shrimp survival administered with prebiotic 0.8% were significantly higher (P<0.05) than control. The administration of prebiotic with dose 0.8% was the best result and could effectively improve the immune responses and growth performance of whiteleg shrimp.
其他关键词:prebiotic; whiteleg shrimp; white spot disease