标题：Isolation and Characterization of Partial Sequence of merA Gene from Mercury Resistant Bacterium Klebsiella pneumonia Isolated from Sario River Estuary Manado
期刊名称：Research Journal of Environmental and Earth Sciences
印刷版ISSN：2041-0484
电子版ISSN：2041-0492
出版年度：2014
卷号：6
期号：3
页码：156-160
DOI：10.19026/rjees.6.5754
出版社：Maxwell Science Publications
摘要：The most common bacterial mercury resistance mechanism is based on the reduction of Hg2+ to Hg0, which is dependent on the mercuric reductase enzyme ( merA ) activity. The aims of this research were to isolate and characterize merA gene fragment of mercury resistant bacteria Klebsiella pneumonia isolate A1.1.1. The gene fragment was amplified by PCR using previously designed primer pairs. Plasmid DNAs were used as template. The result showed that the partial sequence of merA gene has been found on plasmid DNA of mercury resistant bacterium Klebsiella pneumonia isolates A1.1.1. The nucleotide sequence of the merA gene consists of 285 base pairs (bp) which encodes deduced 94 amino acids of mercury reductase merA protein. The merA protein sequence of isolate A1.1.1 has 99% similarity with some strains of Klebsiella pneumonia deposited in Gen Bank. There is a gene mutation that causes the deduced amino acid threonine was replaced by serine at position 524 (ThrSer) in the merA protein of Klebsiella pneumonia as the accession number: AAR91471.1.
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