期刊名称：Journal of Computer Science & Systems Biology
印刷版ISSN：0974-7230
出版年度：2008
卷号：1
期号：1
页码：021-040
DOI：10.4172/jcsb.1000002
出版社：OMICS Publishing Group
摘要：The polymerase chain reaction (PCR) is an enzymatic reaction which follows simple, predictable and well understood principles. Selective amplification of nucleic acid molecules, that are initially present in minute quantities, provides a powerful tool for analyzing nucleic acids. In this context, efficiency and sensitivity of the PCR largely depends on the efficiency of the primers that are employed for the amplification of a concerned gene. Environmental adversities like drought resulting in scarcity of water have detrimental effects on crop yields worldwide. Sustainable agricultural and food productivity requires development of stress resistant plant species like drought resistant crops that can with stand and flourish in scanty water level environments. A key to underlying such attempts is the molecular understanding of the discrete stress processes that are interwoven at multiple levels. In this review, we discuss about some of the contemporary developments in the area of stress resistance by plants along with the various approaches for the PCR primer designing of two key genes involved in drought resistance, DREB1A and DREB2A.
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