文章基本信息

标题：Unbiased classification of mosquito blood cells by single-cell genomics and high-content imaging
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作者：Maiara S. Severo ; Jonathan J. M. Landry ; Randall L. Lindquist 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2018
卷号：115
期号：32
页码：E7568-E7577
DOI：10.1073/pnas.1803062115
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mosquito blood cells are immune cells that help control infection by vector-borne pathogens. Despite their importance, little is known about mosquito blood cell biology beyond morphological and functional criteria used for their classification. Here, we combined the power of single-cell RNA sequencing, high-content imaging flow cytometry, and single-molecule RNA hybridization to analyze a subset of blood cells of the malaria mosquito Anopheles gambiae . By demonstrating that blood cells express nearly half of the mosquito transcriptome, our dataset represents an unprecedented view into their transcriptional program. Analyses of differentially expressed genes identified transcriptional signatures of two cell types and provide insights into the current classification of these cells. We further demonstrate the active transfer of a cellular marker between blood cells that may confound their identification. We propose that cell-to-cell exchange may contribute to cellular diversity and functional plasticity seen across biological systems.
关键词：entomology ; insect immunity ; malaria ; blood cells ; single-cell sequencing