文章基本信息

标题：Isolation and differential transcriptome of vascular smooth muscle cells and mid-capillary pericytes from the rat brain
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作者：Stéphanie Chasseigneaux ; Yasmine Moraca ; Véronique Cochois-Guégan 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：12272
DOI：10.1038/s41598-018-30739-5
语种：English
出版社：Springer Nature
摘要：Brain mural cells form a heterogeneous family which significantly contributes to the maintenance of the blood-brain barrier and regulation of the cerebral blood flow. Current procedures to isolate them cannot specifically separate their distinct subtypes, in particular vascular smooth muscle cells (VSMCs) and mid-capillary pericytes (mcPCs), which differ among others by their expression of smooth muscle actin (SMA). We herein describe an innovative method allowing SMA⁺ VSMCs and SMA⁻ mcPCs to be freshly isolated from the rat cerebral cortex. Using differential RNA-Seq analysis, we then reveal the specific gene expression profile of each subtype. Our results refine the current description of the role of VSMCs in parenchymal cortical arterioles at the molecular level and provide a unique platform to identify the molecular mechanisms underlying the specific functions of mcPCs in the brain vasculature.