文章基本信息

标题：Dietary magnesium deficiency impaired intestinal structural integrity in grass carp (Ctenopharyngodon idella)
本地全文：下载
作者：Shuo-Peng Wei ; Wei-Dan Jiang ; Pei Wu 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：12705
DOI：10.1038/s41598-018-30485-8
语种：English
出版社：Springer Nature
摘要：Grass carp (223.85–757.33 g) were fed diets supplemented with magnesium (73.54–1054.53 mg/kg) for 60 days to explore the impacts of magnesium deficiency on the growth and intestinal structural integrity of the fish. The results demonstrated that magnesium deficiency suppressed the growth and damaged the intestinal structural integrity of the fish. We first demonstrated that magnesium is partly involved in (1) attenuating antioxidant ability by suppressing Nrf2 signalling to decrease antioxidant enzyme mRNA levels and activities (except CuZnSOD mRNA levels and activities); (2) aggravating apoptosis by activating JNK (not p38MAPK) signalling to upregulate proapoptotic protein ( Apaf - 1 , Bax and FasL ) and caspase - 2 , - 3 , - 7 , - 8 and - 9 gene expression but downregulate antiapoptotic protein ( Bcl - 2 , IAP and Mcl - 1b ) gene expression; (3) weakening the function of tight junctional complexes (TJs) by promoting myosin light chain kinase (MLCK) signalling to downregulate TJ gene expression [except claudin - 7 , ZO - 2b and claudin - 15 gene expression]. Additionally, based on percent weight gain (PWG), against reactive oxygen species (ROS), against caspase - 9 and claudin - 3c in grass carp, the optimal dietary magnesium levels were calculated to be 770.38, 839.86, 856.79 and 811.49 mg/kg, respectively.