文章基本信息

标题：Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
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作者：Lu Cao ; Janice Kiely ; Martina Piano 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：12687
DOI：10.1038/s41598-018-30793-z
语种：English
出版社：Springer Nature
摘要：The paper reports a biosensor formed from antibody coated ZnO nano-crystals which has been prepared using a rapid and inexpensive fabrication method which utilises colloidal dispersion enhanced using sonication. This technique was used to prepare highly ordered and uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) using 0.5%, 1% and 5% concentrations of zinc oxide nano-crystal suspensions. Impedance spectroscopy was employed to interrogate the sensor surfaces and confirmed high reproducibility of the fabrication process. Changes in impedance values, at a frequency of 138 Hz, were used to establish dose dependent responses for C-reactive protein (CRP) antigen. A limit of detection of less than 1 ng/ml was demonstrated for nano-surfaces fabricated from concentrations of 1% ZnO.