文章基本信息

标题：Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue
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作者：Justin Christopher D’Antin ; Rafael I. Barraquer ; Francisco Tresserra 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：12739
DOI：10.1038/s41598-018-31178-y
语种：English
出版社：Springer Nature
摘要：In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (H₂O₂) or distilled water (H₂Od),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM H₂O₂ or H₂Od or used as controls, and cultured for one month, during which dark field and tilt illumination photos were taken. These were used to observe and quantify, time until cellular growth and confluence on the posterior capsule. After culture, histological sections were stained for H&E, α-SMA, Ki-67 and vimentin and evaluated. We prevented cellular growth in 50% of H₂Od and 58% H₂O₂ of treated samples. The overall prevention of cell growth compared to cultured controls was significant for both treatments while there was no significant difference between them. In the cases where cellular growth was not prevented, both treatments significantly delay cellular growth. Until day 28 none of the treated samples of either type that had shown growth reached total confluence. All cultured controls reached total confluence before treated samples (median = day 11.5). Also, histologically, there was a clear morphological difference between cultured controls and treated samples.