文章基本信息

标题：Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)
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作者：Vitalijs Zubkovs ; Alessandra Antonucci ; Nils Schuergers 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：13770
DOI：10.1038/s41598-018-31928-y
语种：English
出版社：Springer Nature
摘要：Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000–1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as negligible tissue autofluorescence within this optical range, offers increased resolution and contrast with even greater penetration depths. Here, we present a custom-built spinning-disc confocal laser microscope (SDCLM) that is specific to imaging in the NIR-II. The SDCLM achieves a lateral resolution of 0.5 ± 0.1 µm and an axial resolution of 0.6 ± 0.1 µm, showing a ~17% and ~45% enhancement in lateral and axial resolution, respectively, compared to the corresponding wide-field configuration. We furthermore showcase several applications that demonstrate the use of the SDCLM for in situ , spatiotemporal tracking of NIR particles and bioanalytes within both synthetic and biological systems.