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标题：Differential regulation of chloride homeostasis and GABAergic transmission in the thalamus
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作者：Tobias Schmidt ; Nikoo Ghaffarian ; Camille Philippot 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2018
卷号：8
期号：1
页码：13929
DOI：10.1038/s41598-018-31762-2
语种：English
出版社：Springer Nature
摘要：The thalamus is important for sensory integration with the ventrobasal thalamus (VB) as relay controlled by GABAergic projections from the nucleus reticularis thalami (NRT). Depending on the [Cl⁻]_i primarily set by cation-chloride-cotransporters, GABA is inhibitory or excitatory. There is evidence that VB and NRT differ in terms of GABA action, with classical hyperpolarization in VB due to the expression of the Cl⁻ extruder KCC2 and depolarizing/excitatory GABA action in the NRT, where KCC2 expression is low and Cl⁻ accumulation by the Cl⁻ inward transporter NKCC1 has been postulated. However, data on NKCC1 expression and functional analysis of both transporters are missing. We show that KCC2-mediated Cl⁻ extrusion set the [Cl⁻]_i in VB, while NKCC1 did not contribute substantially to Cl⁻ accumulation and depolarizing GABA action in the NRT. The finding that NKCC1 did not play a major role in NRT neurons is of high relevance for ongoing studies on the therapeutic use of NKCC1 inhibitors trying to compensate for a disease-induced up-regulation of NKCC1 that has been described for various brain regions and disease states like epilepsy and chronic pain. These data suggest that NKCC1 inhibitors might have no major effect on healthy NRT neurons due to limited NKCC1 function.