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标题：The concentration of poliovirus antigens for D-antigen determination
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作者：J. D. van Ramshorst
期刊名称：Bulletin of the World Health Organization
印刷版ISSN：0042-9686
出版年度：1967
卷号：36
期号：2
页码：209-218
语种：English
出版社：World Health Organisation
摘要：A study was made of the concentration procedures which are necessary in order to determine the D-antigen content of poliomyelitis vaccines by means of the gel-diffusion method. The most suitable methods were considered to be the aluminium-phosphate method developed by Fantes, and ultrafiltration. The AlPO₄ method was found to be superior in precision to ultrafiltration, but the latter was more convenient for small samples. The AlPO₄ method usually gave about 10%-20% lower D-antigen values than the filtration method, probably owing to incomplete elution of antigen from the precipitate. A two-step concentration procedure involving AlPO₄ precipitation followed by ultrafiltration was employed for determining the D-antigen content of combined and/or adsorbed vaccines. The importance of the different variables which occur in D-antigen determination by these methods is discussed. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 209 210 211 212 213 214 215 216 217 218