文章基本信息

标题：Evaluation of enterovirus immune horse serum pools for identification of virus field strains
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作者：Nathalie J. Schmidt ; Joseph L. Melnick ; Herbert A. Wenner 等
期刊名称：Bulletin of the World Health Organization
印刷版ISSN：0042-9686
出版年度：1971
卷号：45
期号：3
页码：317-330
语种：English
出版社：World Health Organisation
摘要：Immune horse sera to 42 enterovirus immunotypes were pooled according to the Lim Benyesh-Melnick and the ”intersecting serum” schemes. Each serum was diluted in the pools to contain 50 antibody units. After it was established that the pools correctly neutralized prototype virus strains, they were evaluated in tests against 273 enterovirus field strains representing most of the viral types included in the pools. With test virus doses of 10-100 TCD₅₀, most of the poliovirus and coxsackievirus field strains were correctly identified in both schemes, but a number of the echoviruses were neutralized by heterotypic pools, particularly in the Lim Benyesh-Melnick scheme. However, at higher test virus doses of 320-3200 TCD₅₀, little heterotypic neutralization occurred in either scheme, and 93-94% of the virus field strains were correctly identified in each scheme. With these larger virus doses, breakthrough tended to occur in homologous pools by the 7th day, but rarely by the 5th day. Since the Lim Benyesh-Melnick pool scheme employs 8 pools as compared with 13 for the intersecting serum scheme, and since the two schemes were equally satisfactory for identifying virus field strains at test virus doses of 320-3200 TCD₅₀, immune horse sera will be pooled by the former scheme, thus utilizing fewer pools, for distribution to qualified viral diagnostic laboratories. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 317 318 319 320 321 322 323 324 325 326 327 328 329 330