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标题：Diagnosis of Plasmodium falciparum infection in man: detection of parasite antigens by ELISA
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作者：L. J. Mackey ; I. A. McGregor ; N. Paounova 等
期刊名称：Bulletin of the World Health Organization
印刷版ISSN：0042-9686
出版年度：1982
卷号：60
期号：1
页码：69-75
语种：English
出版社：World Health Organisation
摘要：An ELISA method has been developed for the diagnosis of Plasmodium falciparum infection in man. Parasites from in vitro cultures of P. falciparum were used as source of antigen for the solid phase and the source of specific antibody was immune Gambian sera; binding of antibody in antigen-coated wells was registered by means of alkaline phosphatase-conjugated anti-human IgG. Parasites were detected on the basis of inhibition of antibody-binding. The test was applied to the detection of parasites in human red blood cells (RBC) from in vitro cultures of P. falciparum and in RBC from infected Gambians; RBC from 100 Geneva blood donors served as normal, uninfected controls. In titration experiments, the degree of antibody-binding inhibition correlated with the number of parasites in the test RBC. Parasites were detected at a level of 8 parasites/10⁶ RBC. Samples of RBC were tested from 126 Gambians with microscopically proven infection; significant antibody-binding inhibition was found in 86% of these cases, where parasitaemia ranged from 10 to 125 000/μl of blood. The presence of high-titre antibody in the test preparations was found to reduce the sensitivity of parasite detection in infected RBC from in vitro cultures mixed with equal volumes of different antibody-containing sera. The sensitivity was restored in most cases by recovering the RBC by centrifugation before testing. In a preliminary experiment, there was no significant difference in antibody-binding inhibition using fresh infected RBC and RBC dried on filter-paper and recovered by elution, although there was greater variation in the latter samples. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1012K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 69 70 71 72 73 74 75