摘要:A molecular survey of 230 serum samples from cattle was studied by PCR-amplification of the citrate synthase gene gltA, the gene coding for protein 190 kDa— omp A—and the gene omp B. The study was carried out in the Junta of Castilla y León (northern Spain). The results suggest that the molecular study of the serum cattle would not make a good method in epidemiological studies on rickettsiae in this region. But it is necessary to continue and expand the work with more sensitive molecular methods.