文章基本信息

标题：Design of Slit between Micro Cylindrical Pillars for Cell Sorting
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作者：Yusuke Takahashi ; Shigehiro Hashimoto ; Haruka Hino 等
期刊名称：Journal of Systemics, Cybernetics and Informatics
印刷版ISSN：1690-4532
电子版ISSN：1690-4524
出版年度：2016
卷号：14
期号：6
页码：8-14
出版社：International Institute of Informatics and Cybernetics
摘要：Micro slits have been designed between micro cylindricalpillars to sort biological cells. Micro cylindrical pillars of 0.02mm diameter and 0.055 mm height were fabricated on the glassplate using the photolithography technique. Variation wasmade on the gap between pillars: 0.01 mm, 0.02 mm, 0.03 mm,and 0.04 mm. The micro pillars are set in the flow pathbetween parallel plates, of which dimension of the cross sectionis 5 mm width and 0.055 mm height. Three kinds of biologicalcells were used in the test alternatively: C2C12 (mousemyoblast cell line originated with cross-striated muscle of C3Hmouse), Hepa1-6 (mouse hepatoma cell line of C57L mouse),or swine red blood cell. The suspension of cells wasintroduced into the slits by the syringe pump at the flow ratebetween 0.4 and 500 cm3/hour. The deformation of the cell atthe slit can be observed by the microscope. The experimentalresults show that the designed slit has capability for sortingcells according to the size and deformability of the cell.
关键词：Biomedical Engineering; Cell Sorting; C2C12;Hepa1-6; Red Blood Cell; Photolithography; and Micro Flow;Channel.