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  • 标题:Effect of Ultrasonic Vibration on Proliferation and Differentiation of Cells
  • 本地全文:下载
  • 作者:Haruka Hino ; Shigehiro Hashimoto ; Yusuke Takahashi
  • 期刊名称:Journal of Systemics, Cybernetics and Informatics
  • 印刷版ISSN:1690-4532
  • 电子版ISSN:1690-4524
  • 出版年度:2016
  • 卷号:14
  • 期号:6
  • 页码:1-7
  • 出版社:International Institute of Informatics and Cybernetics
  • 摘要:The effect of mechanical stimulation of vibration onproliferation and differentiation of cells has been studied invitro. To apply the vibration on the cells, a piezoelectricelement was attached on the outside surface of the bottom ofthe culture plate of six wells. The piezoelectric element wasvibrated by sinusoidally alternating voltage at 1.0 MHzgenerated by a function generator. Five kinds of cells wereused in the experiment: C2C12 (mouse myoblast cell), L929(fibroblast connective tissue of mouse), Hepa1-6 (mousehepatoma cell), HUVEC (human umbilical vein endothelialcell), and Neuro-2a (mouse neural crest-derived cell line).After the incubation for 24 hours, cells were exposed to theultrasonic vibration intermittently for three days: for thirtyminutes per day. At the end of the experiment, the number ofcells was counted by colorimetric method with a microplatephotometer. In the case of Neuro-2a, the total length of theneurite was calculated at the microscopic image. Theexperimental study shows following results. Cells areexfoliated by the strong vibration. Proliferation anddifferentiation of cells are accelerated with mild vibration. Theoptimum intensity of vibration depends on the kind of cells.
  • 关键词:Biomedical Engineering; Vibration; C2C12; L929;Hepa1-6; HUVEC and Neuro-2a.
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