文章基本信息

标题：Behavior of Cell in Uniform Shear Flow Field between Rotating Cone and Stationary Plate
本地全文：下载
作者：Shigehiro Hashimoto ; Hiromi Sugimoto ; Haruka Hino 等
期刊名称：Journal of Systemics, Cybernetics and Informatics
印刷版ISSN：1690-4532
电子版ISSN：1690-4524
出版年度：2018
卷号：16
期号：2
页码：1-7
出版社：International Institute of Informatics and Cybernetics
摘要：The behavior of a biological cell in a uniform shear flowbetween the rotating cone and the stationary plate has beenobserved in vitro. The cone and plate apparatus is mounted ona stage of an inverted phase contrast microscope to observecells adhered to the plate under the shear flow. Deformationand migration of cells during exposure to the shear flow for 24hours were traced with the time lapse images. Two kinds ofcells were used in the test: C2C12 (mouse myoblast cell line),and HUVEC (human umbilical vein endothelial cell). Theexperimental results show that HUVEC tends to migratedownstream at the shear stress of 1 Pa. C2C12 tends to tiltvertically in the flow for 18 hours between 1 Pa and 2 Pa.HUVEC tends to be rounded at the shear stress of 2 Pa. Thebehavior of each isolated cell in the shear flow field has beenable to be quantitatively observed by the cone and plateapparatus in vitro.
关键词：Biomedical Engineering; Shear Stress; C2C12;HUVEC and Couette Flow.