文章基本信息

标题：+ -ATPase in Lactobacillus casei Zhang
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作者：Chen X. ; Yang M. ; Sun Z. 等
期刊名称：Czech Journal of Food Sciences
印刷版ISSN：1212-1800
电子版ISSN：1805-9317
出版年度：2009
卷号：27
期号：1
页码：49-54
DOI：10.17221/134/2008-CJFS
出版社：Czech Academy of Agricultural Sciences
摘要：The acid tolerance is an important property of Lactic acid bacteria as potential probiotics. H⁺-ATPase is considered a key gene in several bacteria with the ability of acid tolerance. We cloned and sequenced the full length cDNA of alpha subunit of H⁺-ATPase gene in Lactobacillus casei Zhang, which had been isolated from traditional home-made koumiss in Inner Mongolia of China. The results showed that the respective cDNA sequence is composed of 1530 nucleotides and codes a putative protein including 509 amino acids. In addition, we also reconstructed the phylogenic trees for H⁺-ATPase gene based on amino acids sequences of diverse strains of Lactic acid bacteria.
关键词：H + -ATPase; clone; sequence; Lactobacillus casei Zhang; phylogenic trees