文章基本信息

标题：Cronobacter sakazakii DBM 3157 T and Cronobacter malonaticus DBM
本地全文：下载
作者：Marounek M. ; Putthana V. ; Benada O. 等
期刊名称：Czech Journal of Food Sciences
印刷版ISSN：1212-1800
电子版ISSN：1805-9317
出版年度：2012
卷号：30
期号：6
页码：573-580
DOI：10.17221/433/2011-CJFS
出版社：Czech Academy of Agricultural Sciences
摘要：Cronobacter sakazakii and Cronobacter malonaticus are pathogens causing infections in children that are primarily linked to the consumption of contaminated infant milk formula and food. Both Cronobacter strains examined were susceptible to caprylic acid, monocaprylin and, to a lesser extent, sorbic acid. Capric acid, lauric acid, monosorbin, monocaprin, monolaurin, and sucrose caprate exhibited no inhibitory activity. Caprylic acid and monocaprylin treatment (2 mg/ml) of C. sakazakii DBM 3157^T reduced the number of viable cells by five orders of magnitude. In the case of C. malonaticus DBM 3148, both caprylic acid and monocaprylin (2 mg/ml) decreased the viable cell counts below the limits of detection. The bactericidal activity of monocaprylin increased as a function of concentration (0.5–2.0 mg/ml) and temperature (40–55°C). The exposure of each Cronobacter strain to monocaprylin resulted in the release of cellular proteins and nucleic acids. Electron microscopy revealed that the antimicrobial treatment damaged cytoplasmic structures and resulted in cell aggregation. The combination of monocaprylin at 0.5 mg/ml and increased temperature (50°C) appears to be a suitable treatment against C. sakazakii and C. malonaticus .
关键词：Cronobacter sp.; fatty acids; bacterial activity; monocaprylin