文章基本信息

标题：Cloning and prokaryotic expression of 1-deoxy-D-xylulose-5-phosphate synthase (DXS) from Lepidium apetalum
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作者：Ligang Ma ; Yongguang Han ; Le Zhao 等
期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2018
卷号：170
期号：5
页码：052021
DOI：10.1088/1755-1315/170/5/052021
语种：English
出版社：IOP Publishing
摘要：1-Deoxy-D-xylulose-5-phosphate synthase (DXS) is the first enzyme in plastidial methylerythritol phosphate pathway for isoprenoid biosynthesis. With primers designed according to seedling transcriptome data, we cloned a full-length cDNA fragment of DXS gene from Lepidium apetalum by PCR amplification, and designate it as LaDXS (GenBank accession no. KU314760). The gene contains a 2148-bp ORF and encodes a protein with 715 amino acids. Recombinant LaDXS protein was successful expressed in E.coli BL21 (DE3). Cloning and prokaryotic expression of LaDXS is important for further studies of isoprenoid biosynthesis in L. apetalum.