文章基本信息

标题：Evaluation of the Xenobiotic Effects of Various Stressors in the Hypothalamus and Test is of Laboratory Mouse with Special Reference to GST Gene
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作者：Arun Kumar Yadawa ; Chandra Mohini Chaturvedi
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2017
卷号：6
期号：7
页码：12762
DOI：10.15680/IJIRSET.2017.0607043
出版社：S&S Publications
摘要：The glutathione S-transferase (GST) family of enzymes has a vital role in biotransformation ofenvironmental carcinogens, pollutants, drugs and other xenobiotics. GST M has been found in high concentrations inbrain, muscle, liver, kidney and lung. GST P is most prevalent in mammalian cells, present at high level in manycancerous tissues. Aim of this study was to check the xenotoxicity of various stressors with reference to GST geneexpression. In this experiment both hypothalami and testes of mice receiving different stressors (water and fooddeprivation, BPA, CPA, PQ and C. sativa leaf extract) were processed for the RT-PCR of GST M1 and GST P1. RTPCRresults showed that the expression of GST P1 mRNA increased in the hypothalamus of BPA treated mice andsimilarly expression of GST M1 mRNA increased in the hypothalamus of CPA treated mice only. Accordingly,expression of GST M1 remained unchanged in the hypothalamus and testis of water and food deprived mice, BPA, PQand C. sativa leaf extract treated mice while expression of GST P1 remained unchanged in the hypothalamus and testisof water and food deprived mice, CPA, PQ and C. sativa leaf extract treated mice except in the hypothalamus of BPAtreated mice. From these results it can be concluded that, different stressors used in this study have no xenobioticeffects except BPA and CPA which modulated GST P1 and GST M1 mRNA respectively in the hypothalamus to someextent. However, more study is needed to confirm xenobiotic property of these compounds especially when given athigher dose level and for longer duration.
关键词：Bisphenol A; Cyclophosphamide; GST; Paraquat; Xenotoxicity.