文章基本信息

标题：Plasmatic higher levels of homocysteine in Non-alcoholic fatty liver disease (NAFLD)
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作者：Sylene Coutinho Rampche de Carvalho ; Maria Tereza Cartaxo Muniz ; Maria Deozete Vieira Siqueira 等
期刊名称：Nutrition Journal
印刷版ISSN：1475-2891
电子版ISSN：1475-2891
出版年度：2013
卷号：12
期号：1
页码：37
DOI：10.1186/1475-2891-12-37
语种：English
出版社：BioMed Central
摘要：Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease, which includes a spectrum of hepatic pathology such as simple steatosis, steatohepatitis, fibrosis and cirrhosis. The increased serum levels of homocysteine (Hcy) may be associated with hepatic fat accumulation. Genetic mutations in the folate route may only mildly impair Hcy metabolism. The aim of this study was to investigate the relation between liver steatosis with plasma homocysteine level and MTHFR C677T and A1298C polymorphisms in Brazilian patients with NAFLD. Thirty-five patients diagnosed with NAFLD by liver biopsy and forty-five healthy controls neither age nor sex matched were genotyped for C677T and A1298C MTHFR polymorphisms using PCR-RFLP and PCR-ASA, respectively, and Hcy was determined by HPLC. All patients were negative for markers of Wilson’s, hemochromatosis and autoimmune diseases. Their daily alcohol intake was less than 100 g/week. A set of metabolic and serum lipid markers were also measured at the time of liver biopsies. The plasma Hcy level was higher in NAFLD patients compared to the control group (p = 0.0341). No statistical difference for genotypes 677C/T (p = 0.110) and 1298A/C (p = 0.343) in patients with NAFLD and control subjects was observed. The genotypes distribution was in Hardy-Weinberg equilibrium (677C/T p = 0.694 and 1298 A/C p = 0.188). The group of patients and controls showed a statistically significant difference (p 0.05). The suggested genetic susceptibility to the MTHFR C677T and A1298C should be confirmed in large population based studies.
关键词：Fatty liver ; Non-alcoholic steatohepatitis ; Methylenetetrahydrofolate reductase ( MTHFR ) ; Oxidative stress ; Polymorphisms