文章基本信息

标题：Bacillus sp., B. cereus , and B. licheniformis in raw milk
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作者：Němečková I. ; Solichová K. ; Roubal P. 等
期刊名称：Czech Journal of Food Sciences
印刷版ISSN：1212-1800
电子版ISSN：1805-9317
出版年度：2011
卷号：29
期号：SpecialIssue
页码：S55-S60
DOI：10.17221/313/2011-CJFS
出版社：Czech Academy of Agricultural Sciences
摘要：Totally 75 raw milk samples were analysed with the methods employing the media compared – MYPA, PEMBA, Brilliance^TM Bacillus cereus agar, and HiCrome Bacillus agar. The reference method with MYPA seems to be the most suitable for dairy plants laboratories because there is only low risk of mistaken identity. However, the samples containing miscellaneous micro-flora should be heat-inactivated before plating. Both positive and negative strains (totally 132) were isolated. Twelve strains, which could cause problems in the evaluation of the plates, were selected and identified by phenotyping and by PCR methods for Bacillus sp., B. cereus , and B. licheniformis . The PCR methods differed in their selectivity within particular bacilli group, within genera Bacillus , and within raw milk microflora.
关键词：cultivation method; chromogenic medium; genera-specific PCR; species-specific PCR